The statement came after Corriere della Sera daily reported that the attack earlier in 2026 had emptied the Uffizi's servers and prompted the emergency transfer of valuable jewels to the Bank of Italy.
The Uffizi said it had been targeted by a cyberattack on February 1, but nothing had been stolen and no information was lost.
It also denied that the hackers had obtained security maps or that employees' phones had been infiltrated.
The Uffizi Galleries display some of Italy's most celebrated artwork, including Botticelli's Birth of Venus and Primavera paintings, along with Michelangelo's Doni Tondo.
The museum said the only disruption caused by the attack was linked to the time needed to restore back-ups, adding that it had released a statement about the incident after it had happened.
Corriere reported that hackers had infiltrated the network of the Uffizi, Palazzo Pitti and the Boboli Gardens, had taken control of the photographic server and sent a ransom demand to the personal phone of Uffizi director Simone Verde.
The Uffizi said a full back-up of the photo server existed.
It also said the closure of a section of the Palazzo Pitti and subsequent removal of valuables to the Bank of Italy was tied to renovation work planned in 2025 and had nothing to do with the cyberattack.
The Uffizi said the replacement of its surveillance cameras mentioned in the article had been recommended by the police in 2024.
The upgrade was accelerated after thieves in 2025 targeted Paris's Louvre Museum, stealing jewels worth $US102 million ($A148 million) that were still missing, it said.
"The cameras had been in the process of being replaced for a year. The situation was not at all like the Louvre's. The Galleries did have cameras, but they were analogue and are now digital," the Uffizi said.
In March, three paintings by French masters Pierre-Auguste Renoir, Paul Cezanne and Henri Matisse were stolen from a museum in northern Italy.