Russia launched 324 drones overnight and three ballistic missiles, Ukraine's air force said on Wednesday.
Air defence units shot down or neutralised 309 drones, but the missiles and 13 drones hit at nine locations, it said.
"Just over the past 24 hours there were brutal attacks on Dnipro, Cherkasy, Kharkiv, Kryvyi Rih, Chernihiv, the Donetsk region, and Zaporizhzhia," President Volodymyr Zelenskiy said in a post on X, adding that co-operation to strengthen Ukraine's air defences was a priority.
"We need air defence missiles every single day - every day the Russians continue their strikes on our cities."
On Tuesday, Ukraine and Germany agreed defence co-operation plans and Kyiv also agreed on drone production in Norway.
A 74-year-old woman was killed in a kiosk in a combined attack on the southeastern city of Zaporizhzhia early on Wednesday, regional governor Ivan Fedorov said on the Telegram messaging app.
Fedorov said the attack also damaged a car park, business premises, and nearby residential buildings, as well as an education facility.
Regional prosecutors said the attack on the city involved both drones and missiles.
Three people were injured in an overnight Russian drone attack on the southeastern city of Dnipro, the regional governor, Oleksandr Ganzha, said on the Telegram messaging app.
Ganzha said the attack also damaged an apartment building and an administrative building, posting photos of a building with a gaping hole in one of its sides.
The new attack came after a missile strike on the city killed five and injured close to 30 people on Tuesday.
Four people sought medical attention after an overnight drone attack in the central city of Cherkasy, Ihor Taburets, the regional governor, said.
He said dozens of private homes and cars were damaged.
An earlier drone attack on Tuesday killed an eight-year-old boy and injured another 14 people, Taburets has said.
Port infrastructure on the Danube in Ukraine's southern region of Odesa came under another drone attack, the Ukrainian Sea Ports Authority said, adding that production and storage facilities, as well as an administrative building, were damaged.
Despite attacks, the ports continued to operate, it said.
Officials in the Kyiv region also reported a drone attack.