Andre Van Mason was arrested last Thursday as part of an operation that allegedly led police to a drug lab in Mackenzie, in Brisbane's southeast.
He has now been charged with one count each of murder and unlawful use of a motor vehicle.
He briefly appeared in Brisbane Magistrates Court on Monday, with his matter adjourned until November 7.
A 30-year-old East Brisbane man also charged with murder appeared in court on Saturday.
The victim, a 38 year old man, died after being struck once in the chest at close range at a home in Cliveden Avenue, Oxley in the early hours of Tuesday.
Detective Superintendent Andrew Massingham initially alleged four men and a woman armed with a 12-gauge shotgun, and potentially a handgun, were involved in the attack.
"The male deceased was struck at very close range and we believe the weapon involved was a 12-gauge shotgun," he told reporters on Tuesday.
Police confirmed on Saturday they were still looking for two male suspects as investigations continued into the Inala man's death.
27°C