The technology will be introduced for the first time in Australia by Specialised Breeders Australia, the country's largest supplier of day-old chicks and point-of-lay pullets.
It will be introduced through a partnership with German agri-tech firm Agri Advanced Technologies and its Cheggy machine.
The fully automated Cheggy machine works by shining light through eggs on a conveyor belt and reading the light that passes through.
Because male and female chick embryos develop different colour feathers at an early stage, the machine can tell them apart, without cracking or piercing the shell.
It can scan up to 20,000 eggs per hour.
Male eggs are sorted out and diverted to animal feed and other products, while female eggs continue incubating.
Egg Farmers Australia chief executive officer Melinda Hashimoto said it would be a significant advancement for the industry.
"This advancement represents an important step forward in animal welfare by enabling the identification of the sex of a chick in the early incubation stage, therefore reducing the need for the culling of day-old male chicks," Mrs Hashimoto said.
"It also reflects our industry's ongoing commitment to continuous improvement, innovation, and response to evolving community expectations."
Egg Farmers of Australia acknowledged the large capital investment and leadership shown by SBA in bringing the technology to Australia, and said that in-ovo sexing would play an important role in the sustainable and responsible future of the egg supply chain.
"EFA will continue to work with industry partners, governments and stakeholders to ensure that new technologies are implemented in a practical science-based manner that supports both animal welfare and the long-term viability of egg farming in Australia," Mrs Hashimoto said.
AAT is already the market leader in in-ovo sex determination in Europe, with machines operating in Germany, France, Italy, the Netherlands and Spain, as well as the United States and Brazil.
The Australian installation marks the company's first entry into the Asia-Pacific region.
Aussie egg farmers produce 18.8 million eggs a day from a national flock of 24 million hens. Eggs have the lowest carbon footprint of any animal food protein produced in Australia.
